| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Okongwu, Ijenu U (14)
|
MASERP |
|
29.72 |
- |
| 2 |
Anderson, Caroline E (13)
|
MARAYS |
31.70 |
30.67 |
-1.03 |
| 3 |
Brady, Aisling C (13)
|
MAPCAC |
31.13 |
30.90 |
-0.23 |
| 4 |
Smith, Grace E (14)
|
MARAYS |
33.95 |
31.05 |
-2.90 |
| 5 |
Conaway, Payton M (13)
|
MARAYS |
|
34.67 |
- |
| 6 |
Brown, Paige E (14)
|
MARAYS |
|
34.77 |
- |
| 7 |
Krauss, Natalie (13)
|
MAUN-L |
35.91 |
36.03 |
0.12 |
| 8 |
O'Donnell, Katherine M (13)
|
MANPAC |
|
36.31 |
- |
| 9 |
Hunsberger, Zoe C (13)
|
MANPAC |
|
36.60 |
- |
| 10 |
Bond, Valerie I (14)
|
MAPCAC |
|
36.79 |
- |
| 11 |
Ryan, Juliana R (13)
|
MAHHAA |
36.41 |
37.63 |
1.22 |
| 12 |
McMahon, Fiona M (14)
|
MACHEL |
|
43.26 |
- |
|
Nishikawa, Lauren (14)
|
MAACAC |
|
NS |
- |
|
O'Donnell, Abigail E (13)
|
MAUN-L |
38.51 |
NS |
- |